Journal: Molecular Medicine

Article Title: Induction of Steatohepatitis (NASH) with Insulin Resistance in Wild-type B6 Mice by a Western-type Diet Containing Soybean Oil and Cholesterol

doi: 10.2119/molmed.2016.00203

Figure Lengend Snippet: Dietary cholesterol in soybean oil diet impaired hepatic insulin signaling. Mice received diets as described in Figure 1. Protein levels of (A) insulin receptor substrate 1 (IRS-1) and (B) IRS-2 were determined in liver lysates by Western blot analysis with specific antibodies. Density units were normalized to Fast Green staining, which was verified on the same Western blot membrane as a loading control. (C) Hepatic glycogen content was enzymatically quantified in homogenates (see Materials and Methods section). Values represent means ± standard error of the mean of 5 mice per group. Statistics: One-way analysis of variance with Dunnett’s post hoc test or, where appropriate, Tukey’s post hoc test for multiple comparisons, *p <0.05.

Article Snippet: Western Blot Analysis and Oxyblot Western blot was performed as previously described ( 20 ) with anti-Prx-SO 3 (Abcam, Cambridge, UK), anti-IRS-1 and anti-IRS-2 antibodies (NEB, Frankfurt am Main, Germany) as well as FastGreen staining (Sigma-Aldrich) as a loading control.

Techniques: Western Blot, Staining